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Heres how the equivalence often breaks down: Based on Pod Equivalency Claims: JUUL Labs, for example, has stated that one of its 5% nicotine pods (containing approximately 0.7ml of e-liquid and around 40-48mg of total nicotine, designed for about 200 puffs) is roughly equivalent to one pack of 20 cigarettes

DNA concentration (for samples and controls) was quantified using Quant-iT Picogreen dsDNA Assay (Invitrogen, ThermoFisher Scientific, Carlsbad, CA, USA)

Heated tobacco tends to be simpler than advanced vaping setups, but it still depends on hardware and specific consumables

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